Toxoplasma gondii whole-cell antigen for modified agglutination test (MAT): Toxoplasma gondii is an obligate intracellular parasite that infects mammals and birds. A modified agglutination test (MAT) can be used to detect anti-Toxoplasma IgG antibodies in serum, plasma and other bodily fluid samples of animals. For MAT test, formalin-treated Toxoplasma tachyzoite is used as the antigen. The presence of anti-Toxoplasma IgG antibody in the samples will cause the antigens in suspension to form a mat of cells covering the bottom of a U-shaped well. Samples without pellets at the bottom of wells are positive, samples with pellets are negative.
This whole-cell antigen was prepared using Toxoplasma gondii RH strain cultivated in human foreskin fibroblast cell culture. The tachyzoites harvested from cell culture were treated with formalin, which renders tachyzoites non-viable, non-infective, and non-hazardous.
Storage Condition: 4°C for 6 months
Storage Buffer Composition: Alkaline buffer (7.02g NaCl, 3.09g boric acid (H3BO3), 24 ml of 1N NaOH, 4g of bovine plasma albumin (fraction V) in 1 liter of distilled water, adjust pH to 8.7, and sodium azide (0.1%) as a preservative), final pH 8.2-8.4. Store at 4°C.
- Additional Information on Toxoplasma gondii http://web.utk.edu/~csu1/TgMAT-AG01.html
- Example of MAT test results, see this link: http://web.utk.edu/~csu1/TgMAT-Modified_Agglutination_Test.html
- The recommended protocol for MAT test is provided here: http://web.utk.edu/~csu1/MATprotocol.pdf