Toxoplasma gondii whole-cell antigen for modified agglutination test (MAT): Toxoplasma gondii is an obligate intracellular parasite that infects mammals and birds. A modified agglutination test (MAT) can be used to detect anti-Toxoplasma IgG antibodies in serum, plasma and other bodily fluid samples of animals. For MAT test, formalin-treated Toxoplasma tachyzoite is used as the antigen.

 

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TgMAT Antigen

Toxoplasma gondii whole-cell antigen for modified agglutination test (MAT): Toxoplasma gondii is an obligate intracellular parasite that infects mammals and birds. A modified agglutination test (MAT) can be used to detect anti-Toxoplasma IgG antibodies in serum, plasma and other bodily fluid samples of animals. For MAT test, formalin-treated Toxoplasma tachyzoite is used as the antigen. The presence of anti-Toxoplasma IgG antibody in the samples will cause the antigens in suspension to form a mat of cells covering the bottom of a U-shaped well. Samples without pellets at the bottom of wells are positive, samples with pellets are negative.

This whole-cell antigen was prepared using Toxoplasma gondii RH strain cultivated in human foreskin fibroblast cell culture. The tachyzoites harvested from cell culture were treated with formalin, which renders tachyzoites non-viable, non-infective, and non-hazardous.

Storage Condition: 4°C for 6 months

Storage Buffer Composition:  Alkaline buffer (7.02g NaCl, 3.09g boric acid (H3BO3), 24 ml of 1N NaOH, 4g of bovine plasma albumin (fraction V) in 1 liter of distilled water, adjust pH to 8.7, and sodium azide (0.1%) as a preservative), final pH 8.2-8.4. Store at 4°C.

Information:

*Shipping is only available to the United States and Canada.  For alternative shipping locations please contact UTRF.

**Please allow 2-3 weeks for shipping

Toxoplasma gondii image photo credit.
 

$275.00 – $400.00

TgMAT Kit

Toxoplasma gondii MAT test Kit (TgMAT Kit)
For research only.

Each kit contains:
a). TgMAT antigen, 2.5 ml.
b). Alkaline Buffer, 50 ml.
c). Phosphate Buffered Saline (PBS), 50 ml.
d). Positive control serum, 60 µl
e). Negative control serum, 60 µl
f). Evans blue dye (2 mg/ml in H2O), 1 ml.

*Shipping is only available to the United States and Canada.  For alternative shipping locations please contact UTRF.

**Please allow 2-3 weeks for shipping.

References:

Desmonts G., Remington JS. Direct agglutination test for diagnosis of Toxoplasma infection: Method for increasing sensitivity and specificity. J. Clin. Microbiol. 1980. 11:562-568.

Dubey JP, Desmonts G. Serological responses of equids fedToxoplasma gondii oocysts. Equine Vet. J. 1987. 19:337-339.

 

$350.00