Liposome siRNA Encapsulation (LSE) Kit

$300.00$1,100.00

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Description

Description: Liposome siRNA Encapsulation (LSE) is a liposome assembly technique to enhance siRNA cargo loading into PEGylated neutral liposomes for improved biocompatibility and pharmacokinetics.  This results in liposomal nanocarriers with efficient nucleic acid encapsulation and enhanced cellular association, while minimizing the cytotoxic effects associated with cationic-mediated transfection. LSE is a quick, easy, reproducible, scalable method for nanoparticle-mediated applied transfection.

LSE Kit Components:

  • Composite lipid film 
(1mg per rxn)
  • Lipid-based nucleic acid condenser (1 vial per rxn)
  • Injection buffer (EtOH based buffer, 5ml per 10 rxn)
  • Condensing buffer (Tris-HCl based buffer, 10ml per 10 rxn)

Not included in LSE kit:

  • siRNA sequence for desired gene target
  • dialysis cassette/tubing/etc. for liposome purification – OPTIONAL
  • a quantitative measure for siRNA load (i.e. RiboGreen or other)- OPTIONAL

Storage Condition: 4°C for 1 month


Benefits:

  • One step assembly
  • Eradicates the toxicity due to charged liposomes
  • Reduced nucleic acid leakage
  • Enhanced encapsulation efficiency of the siRNA load
  • Improved cellular association
  • Cost-effective

Inventors:

  • Dr. Deidra J.H. Mountain, Associate Professor,  Department of Surgery in the Division of Vascular and Endovascular Surgery at the University of Tennessee (UT)
  • Richard K. Fisher, Graduate student, UT Graduate school of Medicine
  • Dr. Oscar H. Grandas, Professor, Department of Surgery, Chief,  UT Division of Vascular and Endovascular Surgery

*Shipping is only available to the United States and Canada.  For alternative shipping locations please contact UTRF.