Transgenic Soybean Conferring Tolerance to SCN

Agriculture Technologies for Licensing

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The Technology

Researchers at the University of Tennessee have identified two soybean genes that confer resistance to soybean cyst nematode (SCN). The genes were identified using phylogenetic and microarray analyses, and characterized using biochemical assays. To determine the biological role of the identified genes in soybean resistance against SCN, the researchers generated transgenic hairy roots in susceptible soybean background for individual overexpression of the identified genes. Comparative analyses of the SCN demographics in transgenic hairy roots and control hairy roots showed that the transgenic hairy roots with overexpression of the identified genes in the susceptible soybean background exhibited significant increased resistance to SCN infection. To further understand the defense role of the identified genes in soybean, the researchers compared the expression levels of the identified genes in compatible and incompatible reactions between soybean and soybean mosaic virus. The genes expression was found to be up-regulated in the incompatible reaction, and not so in the compatible reaction. These results indicate that the identified genes play a critical role in soybean defense against SCN and perhaps other diseases. The two identified genes have different mechanisms for resistance to disease causing agents.

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